The team investigates the derailment of a train caused by someone leaving Maj. Mark Wiltshire's Land Rover on the tracks. The army is suspected but Wiltshire was in the officers mess at the time. The team have yet to identify the 21st victim, or determine if that person was on the train or in the car. Working with a survivor's group, they try to re-create where everyone was seated just prior to the crash occurring. When one of the survivors commits suicide, the crash claims its 22nd victim.

The investigation continues and one of the survivors remembers a vital piece of information. It becomes evident that the identification of body 21 may also lead to the cause of the crash. In the end, the solution rests on discovering a an unknown romantic affair and marital betrayal.

Nikki escapes the burning building but Petra dies and Leo's examination shows she was injected with anthrax. Jack identifies one of the basement victims,also injected,as being Lucas Ballinger's wife and Nikki surprises him at the scene of the murders though he flees. He goes after Hearns and later Chrissie finds Roper,who was the accomplice in taking the virus out of Ballinger's workplace, dead. The team must locate Ballinger and find out why he allowed his wife to be a victim as well as stopping him from committing a terrible revenge which could endanger thousands of lives. Leo meanwhile successfully gets Shona's conviction overturned.