Three high school friends gain superpowers after making an incredible discovery underground. Soon they find their lives spinning out of control and their bond tested as they embrace their darker sides.

Spix's Macaws Blu and Jewel and their three children—Bia, Carla, and Tiago—are living happily Rio de Janeiro until Jewel realizes that her children are becoming more like humans. Meanwhile, Blu's former owner, Linda Gunderson and her Ornithology husband, Tulio are on an expedition in the Amazon rainforest and, after a fall down a waterfall, discover a quick-flying Spix's macaw that loses one of its feathers. When word gets out about this through television, Jewel believes that they should go to the Amazon to help find the blue macaws. While the kids are ecstatic, Blu is uncertain, but he is pressured into going along. Rafael, Nico and Pedro decide to come along. Luiz attempts to follow but fails. Blu brings a fanny pack full of supplies, one of which he uses mostly is a Global Positioning System, much to Jewel's displeasure.

When Scrat's acorn sends an asteroid to Earth, the Herd must find a way to stop the asteroid from hitting Earth with the help of a returning friend.